PERMEABILITY OF HYDROPHILIC MODEL DRUGS MODEL DRUGS ACROSS 2/4/A1 INTESTINAL EPITHELIAL CELL MONOLAYERS MODEL DRUGS ACROSS 2/4/A1 INTESTINAL EPITHELIAL CELL MONOLAYERS

MODEL DRUGS ACROSS 2/4/A1 INTESTINAL epithelial CELL MONOLAYERS SUMMARY Investigations of the integrity and broadcast characteristics of 2/4/A1 cadres be possessed of been done in this report. The cell patronage was isolated from rat fetal enteric epithelial cells and transfected with thermolabile SV40 mammoth T antigen. These cells proliferated at 33 °C, unless eliminated the antigen and ceased proliferating at a non-permissive temperature (39°C). At 39°C 2/4/A1 cells started to differentiate except simultaneously the cells also underwent monumental cell death. When cultured at 37°C these cells create confluent and implike monolayers that seemed to have paracellular transport characteristics similar to that of the mankind intestine. Transmission electron microscopy affirm the development of multilayers at 33°C, monolayers at 37°C and defects in the cell layer collect to apoptosis at 39°C. Different immunostainings of ZO-1, E-cadherin and vi nculin confirmed formation of tight and bail junctions. Transepithelial resistance reached a plateau of 25-35 Ohm.cm2, which was similar to the minuscular intestine. In transport studies 2/4/A1 cell line monolayers selectively restricted the permeation of hydrophilic permeability markers proportionate to molecular(a)(a) weight and discriminated more accurately between the molecules of mediocre molecular weight compared to Caco-2 cells. These results indicated that 2/4/A1 cells could be utilize as a model for hydrophilic drug engrossment.

INTRODUCTION The urbane intestine plays a crucial role in the compactness of drugs and nutrients. Exogenous substances cross a series of barriers during the process of en! teral absorption: (1) the aqueous boundary/mucus layer, (2) a supporter layer of epithelial cells, and (3) the lamina propria, which contains the blood and lymph vessels that then transport the clothe drugs to other parts of the body (Artursson 1991). The cell monolayer is comprised of two shoot barriers: the cell membrane and the tight junctions. Most drugs are bring forth up by a passive diffusion across the cell membrane... If you want to get a full essay, wander it on our website: OrderEssay.net

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